The agricultural sector has an important role in the economic development of Ponorogo Regency. As a main livelihood for most of the villagers, farming could cause various safety and health risks for workers, especially caused by pesticide use. The negative effect of using pesticides is that it can reduced levels of cholinesterase enzymes in the blood. This study aims to determine the effect of Age, Length of Work (LoW), Smoking Habit (Smoking), Duration of Pesticide Use a Week (Duration) and Mixing Activities (Mixing) to Blood Cholinesterase Levels (BCL). The research was conducted at Demangan Villaage of Ponorogo Regency. Thirty (30) farmers who have at least 1 year length of work were involved to be a sample that randomly selected. This research using observational analytics method and cross-sectional design to obtain some information from respondent. Multiple linear regression was used to analyze obtained data. The results show that the level of blood cholinesterase among agricultural workers was all normal. The result shows that the predictor variables significantly reduce blood cholinesterase levels by 2.56. Blood Cholinesterase Levels is decreased by Age, Length of Work (LoW), Smoking Habit (Smoking), Duration of Pesticide Use a Week (Duration) and Mixing Activities (Mixing) by 50.5 % and 49,5 % by remaining factors. Mixing activitiesd have the greatest impact on reducing cholinesterase levels in the blood. It could decreases cholinesterase levels by 1.66..