The purpose of this study was to determine differences in the computational thinking skills of first students of the Department of Informatics Engineering based on genders. This study uses quasi-experimental methods. The subjects in this study were semester two students of the Department of Informatics Engineering in 2019/2020. The research subjects were 34 male students and 23 female students. Methods of data collection using tests and observations. The research instrument used was a computational thinking ability test and an observation sheet. Data analysis using a t-test (independent sample) with analysis prerequisites in normality and homogeneity tests. Test analysis utilizing the help of IBM SPSS Statistics 20. The study of research data shows that there are significant differences between the computational thinking ability of male and female students. The average results of the computational thinking ability test showed that male students' computational thinking ability is better than the female students' computational thinking ability. The male and female students' computational thinking skills reach the generalization stage, where male students can find solutions directly through easy-to-understand and straightforward ideas. Meanwhile, female students can explain the flow and concepts used to solve problems.