Fasting is one of the routine worship performed by a Muslim. It has many benefits including controlling lust (Hadith of Bukhari and Muslim). Lust of an organism is controlled by the testosterone hormone. Fasting has the possibility to control the levels of testosterone in the blood so it does not exceed normal levels that be able to manage the organism's libido. The method that is used to determine hormone levels in the blood is the ELISA method (enzymelinked immunosorbent assay). This study aims to determine the fasting effect in the Qur'an on testosterone levels in mice using the ELISA method. This study utilized 5 treatments (n=3). The types of treatments were not fasting (control), fasting in the middle of the Hijri month for 3 days; fasting on Mondays and Thursdays; Dawood fasting, and fasting every day for 36 days. Based on the type of fasting performed, fasting on Monday and Thursdays generated the highest increase in hormone levels followed by Dawood fasting then fasting every day for 36 daysIncreased levels of testosterone did not reach excessive levels; thus it can be concluded that fasting can curb lush in the organism that was accordance with Hadith of Bukhari and Muslim.