Potato (Solanum tuberosum L.) peels contains alkaloids, flavonoids, and polyphenols which are capable of providing antibacterial effects. Potato peels can possibly be made as the preparation of lotion making it easier to be applied use by humans who have skin allergy caused by Staphylococcus aureus bacteria. Therefore, the purpose of this study was to formulate lotion preparation from the ethanol extract of potato peels, test the quality of the lotion preparation from the ethanol extract of potato peels, and observe the activity of the lotion in inhibiting Staphylococcus aureus bacteria. This research was an experiment by producing 3 formulations of lotion preparations which were F1, F2, and F3 using ethanol extract concentration ethanol extract of potato peels 0.8%, 0.9%, and 1%. Potato peels were extracted using maceration by 70% ethanol. The production of the lotion was done by mixing the oil phase (stearate acid, cetyl alcohol, liquid paraffin, perfume) into the water phase (glycerin, triethanolamine, methylparaben, aquadest), and finally became ethanol extract of potato peels. Evaluation of preparations was included pH test and viscosity test according to the Indonesian National Standard (SNI) as well as organoleptic tests, homogeneity tests, and dispersion tests. The antibacterial activity test was carried out using the well diffusion method by observing the inhibitory zone area which was then statistically analyzed using One Way ANOVA with the SPSS 16 statistical program by 95% confidence level. The results showed that the preparations of ethanol extract of potato peels F1, F2 and F3 had the characteristics of light brown, rose and homogeneous. The level of thickness was slightly different, F1 was rather thick, F2 was thick while F3 is very thick. The dispersion test of the F1, F2, and F3 lotions obtained ranged from 6-6.7 cm. The pH test and the viscosity test of lotion F1, F2, and F3 preparations were in accordance with SNI that was pH 7 and viscosity between 30000-34700 cp. The results of the antibacterial activity test based on One Way ANOVA were obtained significant results that was P = 0,000 (P <0.05) with the inhibition zones obtained were F0 (9,625 mm), F1 (12,5 mm), F2 (13,375 mm), F3 (13.5 mm) and K + (16.25 mm). Based on the results of the study, it can be concluded that the ethanol extract of potato peels could be formulated as a preparation for antibacterial lotion. Formulation of the lotion from the ethanol extract of potato peels met the quality requirements of physical testing of lotion which was good in relation to organoleptic test, homogeneity test, and dispersion test as well as SNI 16-4399-1996 on pH test and viscosity test. The preparation of the lotion from the ethanol extract of potato peels formulation F1, F2 and F3 had antibacterial activity against Staphylococcus aureus bacteria. The greatest inhibition is found in the F3 formulation (1% potato peels concentration).