Avocado seeds (Persea americana Mill.) Is one part of the plant that has benefits as a treatment. The seeds are considered by the community as a waste of fact, in these seeds have secondary metabolites which are included in the class of triterpenoidal alkaloids, tannins, flavonoids, and saponins as pharmacological effects (Marlinda et al., 2012). Development of active substances containing bitter substances, covering the bitter taste and to be more durable it is made in the form of chewable tablet formulations, it can be consumed in all circles and make it easier for some people who are difficult to swallow the drug. Filling material on the physical characteristics of chewable tablets as well knowing the good concentration of mannitol for the formulation of avocado seed extract chewable tablets (Persea americana Mill.).This study was an experimental study using parametric data analysis oneway ANOVA. Chewable tablets were formulated as avocado seed extract (15.6%), Aerosil (1%), Mucilago starch 10% (8%), starch (3%), Mg. Stearic (1%), Talk (8%), Citric acid (1%), in 3 variations of formula, F1 (Mannitol 40%), F2 (Mannitol 50%), and F3 (Mannitol 60%), and lactose as add 100%. In this formulation carried out by a wet granulation method. The results of tablet evaluation data have shown that all formulas generally produce all formulas that meet good tablet requirements. The effect of the use of mannitol which has been analyzed statistically in tablet formulations affects increasing the fragility and uniformity of tablet weight, as well as the waste produced. But it does not affect the uniformity of size, and violence. While the formulation of avocado seed extract chewable tablets containing 40% concentration of mannitol in the 1st formula was the best formulation from the test results of the physical characteristics of tablets. Where in 3rd formulation with a concentration of 60% mannitol gets the most preferred taste by respondents. The concentration of mannitol variation that has been used has not been able to produce a sweet taste of tablets by the characteristics of chewable tablets in general, which has a distinctive sweet taste when chewed and is comfortably consumed so as not to leave a bitter taste.