Thesis
Published
ANTIBACTERIAL ACTIVITIES OF GREEN TEA (Camellia sinensis) ETHANOL EXTRACTS ON THE GROWTH OF Staphylococcus epidermidis
Abstract
Staphylococcus epidermidis is a non-pathogenic skin microbiota that can sometimes
cause diseases such as opportunistic infections. Infection can be treated using antibiotics,
but now some bacteria are starting to be resistant to antibiotics due to their overuse. As
an alternative treatment, plants that have antibacterial properties such as green tea. It has
tannin, flavonoid, and catechin compounds which are phenol and alkaloid compounds.
Ethanol is the most common solvent used to extract compounds in plants. The purpose
of this study was to determine the antibacterial activity of ethanol extracts of green tea on
the growth of Staphylococcus epidermidis. This study was an experiment of 6 treatments,
namely negative control (sterile aquades), positive control (chloramphenicol 30 µg), green
tea extracted with 60%, 70%, 80% and 90% ethanol solvents. Extraction used maceration
method for 6 x 24 hours and antibacterial activity test using the disc diffusion method
(Kirby-Bauer). The data obtained were analyzed utilized one way ANOVA with the resulting
significance value p = 0.00 or p < 0.05. The results showed that the ethanol extract of green
tea had antibacterial activity against the growth of Staphylococcus epidermidis. Ethanol
extracts of 60%, 70%, 80% and 90% of green tea had an average inhibitory zone diameter
respectively of 18.45 mm; 19.86 mm; 16.68 mm and 13.58 mm. The concentration of
ethanol extract of green tea which had the most optimal antibacterial activity was ethanol
extract 70% with an inhibition zone diameter of 19.86 mm.
Publication Details
InstitutionUNIVERSITAS DARUSSALAM GONTOR
DepartmentFARMASI
Item ID2418
Deposited27 Mar 2023 04:23