Background: cigarette smoke is one of the source of free radicals. The influence of free radicals can be suppressed through antioxidant administration. The marigold leaves contain phenolic as antioxidants. This study aims to determine the effect of marigold leaves on hemoglobin levels, erythrocytes and hematocrit of the blood of rats exposed to cigarette smoke. Method: the samples were divided into 3 groups (the control group and the treatment group 1 and 2). Each group consists of 10 male Wistar rats, the control group was only given the exposure of cigarette smoke, while the treatment group 1 and 2 were given the exposure to cigarette smoke by giving marigold leaves at a dose of 4.5 grams and 6.3 grams. Blood sampling was carried out on days 0, 7 and 14 through the retro-orbitalis sinus using the Hematocrit capillary, collected into the microtube then measuring the blood sample parameters using the Hematology Analyzer Sysmex KX-21®. Data were analyzed with One Way Anova test, and posthoc the Duncan test. Results:there was a significant effect between the control group against the treatment group 1 and 2 (p<0.05). there were no significant differences betweenmarigold leaves groups with a dose of 4.5 grams and 6.3 in increasing red blood cell profile (p<1.00). Conclusion: there was significant effect of marigold leaf on red blood cell profile expossed cigarette smoking at a dose of 4.5 gram and 6.3 gram. Key words: cigarette smoke, marigold leaves, red blood cell profile