Robusta coffee contains antiseptic substances, namely caffeine, phenols and volatile acids. Caffeine has an important role in the development of immune resistance, while the phenol content has been shown to have bacteristatic and bactericidal properties as an antiseptic, virus, and fungus. Volatile acids have activities that increase the inhibition of bacterial growth. The purpose of this study was to determine the formulation of antiseptic liquid hand soap from Ngebel Ponorogo robusta coffee (Coffea canephora) extract. This research method is an experimental design (true experimental laboratory), with a completely randomized design (CRD) and theoretically analyzed including; organoptic test with criteria (color, texture, aroma and foam), pH, and free alkali and statistically analyzed using software data processing with one way ANOVA method with 95% significance (α= 0.05). The results of the research on the formulation of antiseptic handwashing liquid soap Robusta Coffee (Coffea canephora) extract for organoleptic test the best formulation was F3 with a concentration of 18%, for pH testing it did not meet the 2017 BSNI standard, and for the free alkali test the best was F3 with a result of 0.00196 .