Fatimah, Zohratun (2025) FORMULATION AND QUALITY EVALUATION OF WATERMELON RIND EXTRACT (Citrullus lanatus) AND ANTI BACTERIAL ACTIVITY TEST AGAINS Staphylococcus aureus. S1 Undergraduate thesis, UNIVERSITAS DARUSSALAM GONTOR.

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Abstract

Antibacterial gel has become an alternative for treating acne-prone skin caused by bacteria. Long-term use of antibacterial gels can lead to irritation. One of the natural ingredients has potential as an antibacterial agent is watermelon (Citrullus lanatus) peel. The aim of this research is to evaluate the physical quality of the watermelon peel extract gel preparation and its effect on the growth of Staphylococcus aureus. This study is experimental, involving gel formulation with extract concentrations of 2%, 6%, and 10%, and physical quality tests such as organoleptic evaluation, viscosity (according to SNI 16-4380-1996), pH (according to SNI 06-2588-1996), spreadability test (according to SNI 06-2588-1992), homogeneity, syneresis, and antibacterial activity against Staphylococcus aureus using the well diffusion method. The results show that all gel preparations of watermelon peel extract meet SNI standards. The watermelon rind extract gel preparations are in gel form, with a transparent brown to dark brown color, a characteristic watermelon peel extract odor, and good homogeneity. The pH values range from 5.39 to 5.03, viscosity between 5,110 to 10,530 cPs, spreadability between 5.22 to 6.34, with no syneresis. The antibacterial activity test results show that all formulations have inhibition zone diameters of 0 mm, 6.54 mm, 10.24 mm, and 16.85 mm. The formulation with 10% extract (F3) has the highest inhibition with an inhibition zone diameter of 16.85 mm, categorized as strong. Therefore, it can be concluded that watermelon peel extract gel meets SNI standards and has antibacterial activity against Staphylococcus aureus. The watermelon peel extract gel formulation has the potential as a natural active ingredient alternative to address skin issues caused by Staphylococcus aureus bacteria.

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