The development of information and communication technology has a significant influence on the development of learning media and method. E-learning is a new way of teaching and learning using information and communication technology as a learning system. Interactive multimedia which is more popular as multimedia instructional, is one kind of the learning media that can be developed and implemented through e-learning system. This study aims to analyze the strategies in developing e-learning based multimedia instructional at the Islamic boarding university. This study has used the qualitative method for data collection and analysis. Data collection was carried out through observation and interviews with several stakeholders and lecturers. They were inquired about strategies in developing e-learning based multimedia instructional at Islamic boarding university. The results showed that the strategy in developing e-learning based multimedia instructional at the islamic boarding university was conducted through five processes. The five processes are composed of analysis, design, development, implementation, and evaluation.