Jamu circulating in the society must meet safety and quality standards, one of the requirements is that it does not contain medicinal chemical. The medicinal chemical content that is often added to jamu pegal linu is paracetamol. Paracetamol is a drug that has analgesic and antipyretic properties, which is used to reduce mild pain such as headaches and fever. However, use of paracetamol in inappropriate doses will damage the liver. This study aims to test the paracetamol content found on jamu pegal linu circulating in Mantingan District. The method used in this study is qualitative analysis with TLC(Thin-layer chromatography) and quantitative analysis using HPLC (High-Performance Liquid Chromatography). The samples analyzed consisted of 5 brands of jamu pegal linu circulating in the Mantingan District. The results of qualitative analysis with TLC showed that one of the five samples of jamu contained by paracetamol, namely Sample E, while the other four samples does not contain paracetamol. Sample E has an Rf value of 0.36 which is close to the paracetamol standard, namely 0.36. The results of quantitative analysis of paracetamol levels were obtained using the High-Performance Liquid Chromatography (HPLC) method. The mobile phase used was methanol pa and aquabides with a ratio of 40: 60, and the stationary phase used was a C18 column with an injection volume of 20 µL. Paracetamol content was found in one of the samples with a level of 10%. Keywords: Medicinal Chemical Content, HPLC, Jamu Pegal Linu, TLC, Paracetamol